Ecosystem Science & Management

Achievements & Recognition

• US Patent No. 5,164,310, Smith, Gould & Ulian, Transformation of Plants Via the Shoot Apex
• US Patent of the year, TAES Nominee 1992
• Sigma Xi, Science & Engineering Honorary Society 1983
• Gamma Sigma Delta, Agriculture Honorary Society 1997
• Biography in Marquis Who’s Who in Science and Engineering, 2nd Ed. 1989; Who’s Who in the World, 18th Ed. 2001
• Athletic Letter, awarded by Dept. Intercolligiate Athletics, Univeristy of Illinois 2004
• Silver (1) & Bronze (2) Medalist, Womens Swimming T2i, Paralympic Games Tokyo 1964
• Gold Medalist (3), Womens Swimming T2i, Stoke-Mandeville International Games UK 1963 & 1965

Research Areas

Genotype-Independent Plant Transformation using Agrobacterium: Conifers, Cotton, the Cereals and grasses. Regulation of Plant Development: Reduction of gossypol synthesis in cotton seed; cell division/elongation, cellulose & secondary wall synthesis; terminal differentiation in the cotton fiber model.

Bioremediation : Risk assessment of transgenic plants developed for remediation of contaminated sites and polluted habitats.

Current Funding

2003/2004 I-4 Breeder Driven Molecular Program for Monocots

Hatch TEX 08458: Enhancement of Plant Transformation & gene Expression 1997-P

Relevant Past Funding

Newton, Gould 1993-1997: Plant Genome Program NRI, $153,000, Conifer Transformation using Agrobacterium and shoot apices.

Gould, Smith NISSHINBO Co., Inc. 1988-93. $280-000 Comparative study of unbound ABA & IAA in developing fiber cells and embryos of Gossypium barbadense & G. hirsutum.

Gould, Wild, Cobb, Newton: US EPA# C R 822874-01-1/RF 8833, Risk Assesment of Biotechnology Products

Gould: THECB 000517-0089-1997 Transformation of Texas Crops for Insect Resistance $178,000 1998-00

Gould Cotton Inc.: 99-700 Transgenic Approach to Reduce Cotton Seed Gossypol $25,000 1999-04

Keller, Gould: TexCot Effects of Anti-Fungals on Aflatoxin Production and Fungal Diseases of Cotton $75,000 1996-00

Recent Publications

Gould JH, Zhou Y, Padmanabhan V, Magallenes-Cedeno M, Newton RJ 2002. Transformation and regeneration of loblolly pine : shoot apex inoculation with Agrobacterium. Mol Breeding 10:131-141.

Li H, Luo J, Hemphill J, Wang JT, Gould JH. 2001. A rapid and high yielding DNA miniprep for cotton (Gossypium spp.) Plant Molecular Biology Reporter 19:1-5.

Luo J & Gould JH 1999. In vitro shoot tip grafting improves recovery of cotton plants from culture. Plant Cell, Tissue & Organ Culture 57:211-213

Gould JH, Magallanes-Cedeno M. 1998. Adaptation of Shoot Apex Agrobacterium Inoculation & Culture to Cotton Transformation. Plant Mol. Biol. Rept. 16 (3) 284-289

Zhou Y, Newton RJ, Gould J. 1997 A Simple Method for Identifying Plant/T-DNA Junction Sequences Resulting from Agrobacterium-mediated DNA Transformation. Plant Mol Biol. Rept. 15:246-254.

Gould JH. 1997. Transformation of the Cereals using Agrobacterium. In: RS Tuan (Ed), Methods in Molecular Biology, Humana Press Inc., Totowa NJ. Vol. 62:489-499.

Mei B, No EG, McWilliams T, Gould JH, Newton RJ. 1997. In vitro regeneration of fourwing saltbush [Atriplex canescens (Pursh) Nutt.] J. Range Manage. 50:413-418.

Gould JH, Devey M, Hasegawa O, Ulian EC, Peterson G, Smith 1991. Transformation of Zea Mays L., using Agrobacterium tumefaciens and the shoot apex. Plant Physiol. 95:426-434.

Gould JH, Banister S, Fahima M, Hasegawa O, Smith RH. 1991b. Regeneration of Gossypium hirsutum and G. barbadense from the shoot apex. 1991. Plant Cell Rept. 10:12-16.

Gould JH, Slater H, Berdasco M, Smith RH. 1990. Late cotton fiber development-IAA. National Cotton Council of America. Beltwide Cotton Prod. Res. Conf. Nat. Cotton Council, Memphis TN Pp 624-625.

Veluthambi K, Krishnan M, Gould J, Smith R, Gelvin S. 1989. Opines stimulate induction of the vir genes of Agrobacterium tumefaciens Ti plasmid. J. Bact. 171:3696-3703.

Smith RH, Gould JH, Ulian EC. 1992. Transformation of plants via the shoot apex. US Patent No. 5,164,310.

Smith RH, Gould JH. 1989. Introductory Essay for Toshio Murashige & Folke Skoog (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures. In: J. Janick (Ed), Classic Papers in Horticultural Science, Am. Soc. Horticultural Sci., Prentice Hall, NJ. Pp 59-63.

Gould JH, Smith RH. 1989. A rapid, non-destructive GUS assay in tissue culture media. Plant Mol Biol. Reporter 7:209-216.

Gould JH, Smith RH. 1989. Transformation systems for corn. Proceedings: 44th Annual Corn & Sorghum Res. Conf. Am. Seed Trade Assoc., NY, NY. Pp 1-10.

Ulian E, Smith RH, Gould JH, McKnight TD. 1988. Transformation of plants via the shoot apex. In Vitro, Cell Devl. Biol. 24:951-954.

Gould J, Smith RH. 1988. A shoot apex method for cotton transformation. Proc. Beltwide Cotton Prod. Res. Conf. Nat. Cotton Council, Memphis TN. Pp 113-114

Downloadable Research Publications

• Cotton DNA Mini-Prep, Li et al., 2001
• Loblolly Pine Transformation, Gould et al., 2002
• Cotton Transformation-PMBR Gould & Magallanes, 1998
• In vitro shoot tip grafting improves recovery of cotton plants from culture, Luo J, Gould JH. 1999
• Regeneration of Cotton, Gould et al., 1991
• Maize Transformation by Agrobacterium, Gould et al.,1991
• Plant T-DNA Border Junction Analysis, Zhou et al., 1997
• Loblolly LP3 Promoter Study, Wang et al., 2003
• Berberine Accumulation in vitro, Gould & Murashige, 1985
• Cotton Fiber Protoplasts & Cell Wall Synthesis, 1986
• Cotton Ovule epidermal Protoplasts & Cell Wall Synthesis, 1986
• Transformation of Plants Via the Shoot Apex, TAMUS US Patent 1992